Work Sheet 9

 

Enzymes: Catalysis and Kinetics

 

1.      Describe the following terms associated with enzymes: catalysis, substrate, reaction specificity, rate-limiting step, coenzyme and prosthetic group.

 

 

 

 

2.      Define the following six types of enzyme reactions: transferase, hydrolase, oxidoreductase, lyase, ligase and isomerase.

 

 

 

 

3.      Define the following terms related to the energy of enzyme reactions: transition state, energy of activation, exergonic, endergonic and free energy change.

 

 

 

 

4.      Discuss the concepts of substrate affinity, Km and apparent Km.

 

 

 

 

 

 

5.      Use the Lineweaver-Burke plot to demonstrate Vmax, Km, competitive inhibition and non-competitive inhibition.

 

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