Nelson’s
Assay:
1. Mix,
well, 0.5 mL of Nelson’s A with 12.5 mL Nelson’s B.
2. Add
1 mL of the mixture (1.) to all tubes.
3. Heat
in boiling H2O bath for 20 min.
4. For
rapid cooling, remove test tubes to a beaker of cold tap water.
5. Add
1 mL of Arsenomolybdate reagent to each tube.
6. Vortex
until any solid Cu2O is dissolved.
7. Read
at 540 nm.
8. Now
add 6 mL of dH2O to each tube.
9. Read,
AGAIN, at 540 nm.