Biochemistry Laboratory II, CHE 453
Monday and Wednesday 1:00-3:50 PM, NSM C-339
Instructor:
Office Hours: TBA.
Prerequisites: (a) CHE 451L and (b) concurrent enrollment in CHE 452
Experimental Protocol Glucose-1-Phosphate Characterization
Experimental
Protocol
Experimental
Protocol
One lab report for each experiment is required and will be graded for content,
discussion of theory and instrument components, quality of data, correctness
of calculations, closeness of experimental results to literature values, justifications
for poor results, discussion of error, and as outlined in the scoring rubric.
Potato Scoring Rubric (COVID-19 Modified)
Wheat Germ Scoring Rubric (COVID-19 Modified)
Written Quiz:
Will cover material from both of the laboratories (100 pts). Types of material
to emphasize: What reaction does wheat germ acid phosphatase catalyze? What
does 7-min hydrolysis followed by the Fiske-Subbarow assay tell us? What do
the results of the Biuret assay tell you in the wheat germ lab? vs the results
of the phosphatase assay? What is phenyl mercuric nitrate used for in the
potato lab?
Writing Assignment: A paper word-processed, double-spaced, 250 words or less, addressing the following tasks is due on Wednesday, May 6th, 2020 (50 pts).
Unexpected challenges in our lives sometimes bring out the best in us. For example, we act heroically or courageously in emergencies, we are charitable when disasters affect our friends, or we are unselfish and patient when misfortune strikes our friends or relatives. Pick an instance in your own life when such a challenge stimulated in you a response that pleased you. Describe the situation, explain how you responded, and tell what you learned about yourself from the experience.
This paper will be judged on rhetoric,
how effectively you address the writing tasks and syntax, grammar.
Grading Criteria: Letter grades are based on the following point distribution. Grades are
criterion-referenced not norm-referenced; each student has an equal opportunity
meet the criteria charted below:
| Lab Reports | 200 pts |
| Quiz | 100 pts |
| Writing Assignment | 50 pts |
| Total | 350 pts |
Grade |
Percentage | Grade | Percentage |
| A | 100-91 | A- | 90-89 |
| B+ | 88-87 | B | 86-81 |
| B- | 80-79 | C+ | 78-77 |
| C | 76-71 | C- | 70-69 |
| D+ | 68-67 | D | 66-60 |
| F | 59-0 |
| Day | Date | Lab Notes & Hand-Outs* | |
| Check-In / Introduction | W | 1/29 | |
| Glucose-1-Phosphate / Wheat Germ Discussion | W | 1/29 |
|
|
Group I:
Glucose-1-Phosphate, Isolation Group II: Wheat Germ, Isolation |
M | 2/03 | Day 1 |
|
Group I:
Glucose-1-Phosphate, Isolation Group II: Wheat Germ, Isolation |
W | 2/05 | Day 2 |
|
Group I:
Glucose-1-Phosphate, Isolation Group II: Wheat Germ, Kinetics |
M | 2/10 | Day 3 |
|
Group I:
Glucose-1-Phosphate, Isolation Group II: Wheat Germ, Kinetics |
W | 2/12 | |
HOLIDAY |
M | 2/17 | |
|
Group II: Wheat Germ, Kinetics |
W | 2/19 | |
|
Group II: Wheat Germ, Kinetics |
M | 2/24 | |
|
Group I:
Glucose-1-Phosphate, Isolation Group II: Wheat Germ, Kinetics |
W | 2/26 | |
|
Group I:
Glucose-1-Phosphate, Characterization Group II: Wheat Germ, Kinetics |
M | 3/02 | Day 8 |
|
Group I:
Glucose-1-Phosphate, Characterization Group II: Wheat Germ, Kinetics |
W | 3/04 | Day 9 |
|
Group I:
Glucose-1-Phosphate, Characterization Group II: Wheat Germ, Kinetics |
M | 3/09 | |
|
Group I:
Group II: Wheat Germ, Kinetics |
W | 3/11 | |
|
Group I: Utility Day (Finish G-1-P) Group II: Utility Day (Finish Wheat Germ) |
M | 3/16 | |
|
LOCKER SWITCH DAY! Group I:
Wheat Germ, Isolation Group II: Glucose-1-Phosphate, Isolation |
W | 3/18 | Day 1 |
|
Group I:
Wheat Germ, Isolation Group II: Glucose-1-Phosphate, Isolation |
M | 3/23 | Day 2 |
|
Group I:
Wheat Germ, Isolation Group II: Glucose-1-Phosphate, Isolation (Lab Report Due, 100 Pts)
|
W | 3/25 | Day 3 |
| HOLIDAY | M | 3/30 | |
| HOLIDAY | W | 4/01 | |
|
Lab Report Due 12 noon, 100 Pts Group I:
Wheat Germ, Kinetics Group II: Glucose-1-Phosphate, Isolation |
M | 4/06 | Day 4 |
|
Group I:
Wheat Germ, Kinetics Group II: Glucose-1-Phosphate, Isolation |
W | 4/08 | Day 5 |
|
Group I:
Wheat Germ, Kinetics Group II: Glucose-1-Phosphate, Isolation |
M | 4/13 | Day 6 |
|
Group I:
Wheat Germ, Kinetics Group II: Glucose-1-Phosphate, Isolation |
W | 4/15 | Day 7 |
|
Group I:
Wheat Germ, Assays
Group II: Glucose-1-Phosphate, Isolation |
M | 4/20 | Day 8 |
|
Group I:
Wheat Germ, Kinetics Group II: G-1-P, Characterization |
W | 4/22 | Day 9 |
|
Group I:
Wheat Germ, Kinetics Group II: G-1-P, Characterization |
M | 4/27 | Day 10 |
|
Group I:
Wheat Germ, Kinetics III
Group II: G-1-P, Characterization |
W | 4/29 | Day 11 |
|
Group I: Utility Day (Finish G-1-P) Group II: Utility Day (Finish Wheat Germ) |
M | 5/04 | Day 12 Lab Report Notes |
| QUIZ (100 Pts); Paper Due, 50 Pts; Lab Report Due 12 noon, 100 Pts | W | 5/06 |